Jonathan Bradfield, Bioinformatics Specialist

Dr. Pandey is a Senior Scientist at CAG, and joined us in September, 2010. He is currently working on several projects – Characterization of Type-1 diabetes susceptibility gene Clec16a in Natural killer cells, Understanding the molecular mechanism and pathogenesis of EPHB4 in Lymphangiomatosis and DcR3 modulation in IBD.

Rahul received his B.Sc. from Poorvanchal University in Jaunpur, and his M.Sc. in Molecular Biology & Biotechnology (MBBT) from Tezpur University in Assam. He went on to complete his doctoral research at the Sanjay Gandhi Post Graduate Institute of Medical Sciences in Lucknow. He completed his PhD thesis, entitled “Potential Immunomodulators of Natural Origin: Molecular Characterization of Mode of Action” in January 2005 at the Sanjay Gandhi Postgraduate Institute of Medical Sciences, in Lucknow, India. In that work, he identified a plant-derived molecular species that interrupted NF-kB activation and blocked NK cell cytotoxicity. NK cells are major contributors to the antiviral and anti-cancer immune responses, and are critically important cells in intact host defense. He started studying NK cell biology as a graduate student at the Sanjay Gandhi Institute of Medical Sciences in Lucknow, India, and has almost 14 years of experience in this field.

Rahul undertook a Postdoctoral Fellowship with Dr. Jordan Orange at CHOP, one of the leading investigators in immunodeficiency research and research on the signaling processes of natural killer cells. Upon joining Dr. Orange’s laboratory in 2005 he extended his studies of NF-kB by studying NK cells from patients with a primary immunodeficiency disease caused by a genetic mutation in the gene encoding a key regulator of NF-kB, named NEMO. He identified several important defects in NK cells from patients with NEMO mutations. In addition, he also studied NK cells from patients with genetic defects in the Wiskott-Aldrich Syndrome protein (WASp). His study on these patient samples has defined a novel pathway by which a cytokine, IL-2, can restore the function of WASp-deficient NK cells. These studies have significant implications in understanding how to overcome the immunodeficiency defect in Wiskott-Aldrich Syndrome patients, and thereby represents a promising treatment strategy.

He has received two prestigious scientific development awards, the 2006 and 2009 Strategic Training in allergy Research (S*TAR) Program award of the AAAAI. These awards included participation in an elite program of developing independent scientists (30) at the AAAAI annual meeting in those years.

He has made both clinical and basic science contributions and have published in The Journal of Experimental Medicine (2007), Journal of Allergy and Clinical Immunology (2008), Journal of Immunology (2007, 2009, 2015), PLoS Genetics (2011), J Clinical Investigation (April 2011) and the Journal of Clinical Investigation (Oct 2012) & Nat Commun. 2015.


Key Publications

Association of CLEC16A with human common variable immunodeficiency disorder and role in murine B cells.
Li J, Jørgensen SF, Maggadottir SM, Bakay M, Warnatz K, Glessner J, Pandey R, Salzer U, Schmidt RE, Perez E, Resnick E, Goldacker S, Buchta M, Witte T, Padyukov L, Videm V, Folseraas T, Atschekzei F, Elder JT, Nair RP, Winkelmann J, Gieger C, et al.
Nat Commun. 2015 Apr 20;6:6804. doi: 10.1038/ncomms7804.

Human immunodeficiency-causing mutation defines CD16 in spontaneous NK cell cytotoxicity.
Grier JT, Forbes LR, Monaco-Shawver L, Oshinsky J, Atkinson TP, Moody C, Pandey R, Campbell KS, Orange JS.
J Clin Invest. 2012 Oct;122(10):3769-80. doi: 10.1172/JCI64837. Epub 2012 Sep 24.

A genome-wide meta-analysis of six type 1 diabetes cohorts identifies multiple associated loci.
Bradfield JP, Qu HQ, Wang K, Zhang H, Sleiman PM, Kim CE, Mentch FD, Qiu H, Glessner JT, Thomas KA, Frackelton EC, Chiavacci RM, Imielinski M, Monos DS, Pandey R, Bakay M, Grant SF, Polychronakos C, Hakonarson H.
PLoS Genet. 2011 Sep;7(9):e1002293. doi: 10.1371/journal.pgen.1002293. Epub 2011 Sep 29.

IL-2 induces a WAVE2-dependent pathway for actin reorganization that enables WASp-independent human NK cell function.
Orange JS, Roy-Ghanta S, Mace EM, Maru S, Rak GD, Sanborn KB, Fasth A, Saltzman R, Paisley A, Monaco-Shawver L, Banerjee PP, Pandey R.
J Clin Invest. 2011 Apr;121(4):1535-48. doi: 10.1172/JCI44862. Epub 2011 Mar 7.

Rapid up-regulation and granule-independent transport of perforin to the immunological synapse define a novel mechanism of antigen-specific CD8+ T cell cytotoxic activity.
Makedonas G, Banerjee PP, Pandey R, Hersperger AR, Sanborn KB, Hardy GA, Orange JS, Betts MR.
J Immunol. 2009 May 1;182(9):5560-9. doi: 10.4049/jimmunol.0803945.

IKBKG (nuclear factor-kappa B essential modulator) mutation can be associated with opportunistic infection without impairing Toll-like receptor function.
Salt BH, Niemela JE, Pandey R, Hanson EP, Deering RP, Quinones R, Jain A, Orange JS, Gelfand EW.
J Allergy Clin Immunol. 2008 Apr;121(4):976-82. doi: 10.1016/j.jaci.2007.11.014. Epub 2008 Jan 7.

Cdc42-interacting protein-4 functionally links actin and microtubule networks at the cytolytic NK cell immunological synapse.
Banerjee PP, Pandey R, Zheng R, Suhoski MM, Monaco-Shawver L, Orange JS.
J Exp Med. 2007 Oct 1;204(10):2305-20. Epub 2007 Sep 4.

NKp30 ligation induces rapid activation of the canonical NF-kappaB pathway in NK cells.
Pandey R, DeStephan CM, Madge LA, May MJ, Orange JS.
J Immunol. 2007 Dec 1;179(11):7385-96.

Book Chapters:

Review: Genes Involved in Type 1 Diabetes: An Update.
Marina Bakay, Rahul Pandey and Hakon Hakonarson.
Genes 2013, 4(3), 499-521; doi:10.3390/genes4030499 16 September 2013.

Comparative Genetic Analysis of Type 1 Diabetes and Inflammatory Bowel Disease, Type 1 Diabetes - Pathogenesis, Genetics and Immunotherapy.
Marina Bakay, Rahul Pandey and Hakon Hakonarson (2011).
David Wagner (Ed.), ISBN: 978-953-307-362-0, InTech,